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Angiogenesis Models - AngioMouse®

Example: Whole-body image of vessels in PC-3-GFP human prostate cancer in nude mouse.

Example: Intravital image of ANIP-973-GFP human lung cancer in nude mouse.

Example: Whole-body image of Lewis lung-GFP mouse carcinoma in nude mouse.

Features

Visualization of tumor vessels using GFP tumor expression

Intravital imaging of tumor vessels

Whole-body imaging of tumor vessels

Quantitation of vessel density

Real-time angiogenesis studies

Angiogenesis drug discovery and evalation

Orthotopic models of all tumor typesites

Whole-body images of time course of microvessel development in orthotopic model of MDA-MB-435-GFP human breast cancer.

EXTERNAL IMAGING OF MICROVESSELS OF GFP-EXPRESSING HUMAN PANCREATIC CANCER GROWING ON THE NUDE MOUSE PANCREAS VISUALIZED THROUGH A REVERSIBLE SKIN FLAP.

A - mouse imaged in light box, B - mouse imaged on dissecting microscope.

BIBLIOGRAPHY

1. Yang, M., Li, L., Jiang, P., Moossa, A.R., Penman, S., and Hoffman, R.M. Dual-color fluorescence imaging distinguishes tumor cells from induced host angiogenic vessels and stromal cells. Proc. Natl. Acad. Sci. USA 100, 14259-14262, 2003.

2. Amoh, Y., Li, L., Yang, M., Moossa, A.R., Katsuoka, K., Penman, S., and Hoffman, R.M. Nascent blood vessels in the skin arise from nestin-expressing hair follicle cells. Proc. Natl. Acad. Sci. USA 101, 13291-13295, 2004.

3. Yang, M., Reynoso, J., Jiang, P., Li, L., Moossa, A.R., and Hoffman, R.M. Transgenic nude mouse with ubiquitous green fluorescent protein expression as a host for human tumors. Cancer Research 64, 8651-8656, 2004.

4. Hoffman, R.M. The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer 5, 796-806, 2005.

5. Amoh, Y., Li, L., Yang, M., Jiang, P., Moossa, A.R., Katsuoka, K., and Hoffman, R.M. Hair follicle-derived blood vessels vascularize tumors in skin and are inhibited by doxorubicin. Cancer Res. 65, 2337-2343. 2005.

6. Amoh, Y., Yang, M., Li, L., Reynoso, J., Bouvet, M., Moossa, A.R., Katsuoka, K., and Hoffman, R.M. Nestin-linked green fluorescent protein transgenic nude mouse for imaging human tumor angiogenesis. Cancer Res. 65, 5352-5357, 2005.

7. Hoffman, R.M., and Yang, M. Color-coded fluorescence imaging of tumor-host interactions. Nature Protocols 1, 928-935, 2006.

8. Amoh, Y., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., Hoffman, R.M., Bouvet, M. Dual-color imaging of nascent blood vessels vascularizing pancreatic cancer in an orthotopic model demonstrates antiangiogenesis efficacy of gemcitabine. J. Surgical Research 132, 164-169, 2006.

9. Amoh, Y., Nagakura, C., Maitra, A., Moossa, A.R., Katsuoka, K., Hoffman, R.M., and Bouvet, M. Dual-color imaging of nascent angiogenesis and its inhibition in liver metastases of pancreatic cancer. Anticancer Research 26, 3237-3242, 2006.

10. Amoh, Y., Bouvet, M., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., and Hoffman, R.M. Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Clin. Exp. Metastasis 23(7-8), 315-322, 2006.

11. Amoh, Y., Li, L., Katsuoka, K., Bouvet, M., and Hoffman, R.M. GFP-expressing vascularization of Gelfoam as a rapid in vivo assay of angiogenesis stimulators and inhibitors. BioTechniques 42, 294-298, 2007.

12. Ji, Y., Hayashi, K., Amoh, Y., Tsuji, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer. Anticancer Res. 27, 713-718, 2007.

13. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Amoh, Y., Hoffman, R.M., and Bouvet, M. Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. J. Surg. Res., Epub ahead of print, 2007.