IN VIVO GFP TUMOR MODELS
WHOLE-BODY IMAGING OF
ORTHOTOPIC PC-3-GFP IN NUDE MICE
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Bladder, human |
Breast cancer, human |
Colon cancer, human |
Cell
type-HTB-9 UC-14
KU-7-GFP human
bladder carcinoma growing on nude mouse bladder

MX-1-GFP and –RFP
breast carcinoma growing on mammary fat pads of SCID
mouse. Whole-body image.
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Cell types -
MDA-MB-435,MDA-MB-231,MDA-MB-468,MCF-7,MX-1
Primary breast tumor MDA-MB-435 and
lymph node metastases expressing GFP or RFP
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Cell types -
HCT-116,COLO-205,SW-620,Ls-180 ,HCT-15 ,KM-12, WiDr,
HT-29,CaCo-2,RKO
AC3488-GFP-tumor
growing on colon and metastasized to liver and lymph
nodes of nude mouse.Whole-body image.. Primary tumor
- red arrow, liver metastases - white arrow, lymph
node - yellow arrow.
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Larynx, human |
Lung cancer, human |
Melanoma, human |
Cell type- Hep-2
Primary tumor growth of Hep-2
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Cell types -
H-460,HOP-62,EKVX,Anip-973,A549
H-460-GFP human lung carcinoma
growing on lung of nude mouse. Whole-body image.
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Cell types - LOXMIVI,SK-mel-5
Human LOX-1-GFP melanoma metastasized
to lung (A), lymp node (B), bone (C) and adrenal
gland (D) of nude mouse.
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Pancreas, human |
Prostate, human |
Stomach, human |
Cell types -
Bx-PC-3,MIA-PaCa-2,
ASPC -1,Cfpaca,PaCa-1, PaCa28
BxPC-3-GFP pancreatic carcinoma
growing on pancreas with liver and spleen
metastasis. Whole-body image. White arrows - primary
tumor, red arrow - spleen metastasis, yellow arrow -
liver metastasis.
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Cell types -
PC-3,DU-145,LnCaP,MDA PCa 2b
PC-3 prostate cancer. White solid
arrows: Primary tumor; white hollow arrows:
mesenteric lymph nodes; pink arrows: inguinal lymph
nodes, green arrow: axial lymph node
 
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Cell types- NUGC-4
NUGC-4-GFP human
stomach tumor in nude mouse. Primary tumor- red
arrow, lymph node metastases - white arrows
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CNS, human |
Fibrosarcoma, human |
Hepatoma, human |
Cell types - SNB-19,324,U87-MG |
Cell types - HT-1080 |
Cell
types - SMMC-7721 |
Melanoma, mouse |
Muscle, mouse |
Osteosarcoma |
Cell types - B16-F10,B16F0 |
Cell types - C2C12 |
Cell types -
OST,L-SLM |
Ovarian, human |
Ovarian, hamster |
Renal, human |
Cell types - OVCAR-8,RGMI-186,
SKOV-3 |
Cell types - CHOK-1 |
Cell types - SN12C, A498 |
Tounge, human |
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Cell types - SCC-25 |
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GFP Publications
1. Chishima, T., Miyagi, Y., Wang, X., Yamaoka, H.,
Shimada, H., Moossa, A.R., and Hoffman, R.M. Cancer invasion and
micrometastasis visualized in live tissue by green fluorescent protein
expression. Cancer Res. 57, 2042-2047, 1997.
2. Chishima, T., Miyagi, Y., Wang, X., Baranov, E., Tan, Y., Shimada, H.,
Moossa, A.R., and Hoffman, R.M. Metastatic patterns of lung cancer
visualized live and in process by green fluorescent protein expression.
Clinical & Experimental Metastasis 15, 547-552, 1997.
3. Chishima, T., Miyagi, Y., Wang, X., Tan, Y., Shimada, H., Moossa, A.R.,
and Hoffman, R.M. Visualization of the metastatic process by green
fluorescent protein expression. Anticancer Res. 17, 2377-2384, 1997.
4. Chishima, T., Yang, M., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Shimada,
H., Moossa, A.R., Penman, S., and Hoffman, R.M. Governing step of metastasis
visualized in vitro. Proc. Natl. Acad. Sci. USA 94, 11573-11576, 1997.
5. Chishima, T., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Yang, M., Shimada,
H., Moossa, A.R., and Hoffman, R.M. The use of histoculture and green
fluorescent protein to visualize tumor cell host interaction. In Vitro Cell.
Dev. Biol. 33, 745-747, 1997.
6. Yang, M., Hasegawa, S., Jiang, P., Wang, X., Tan, Y., Chishima, T.,
Shimada, H., Moossa, A.R., and Hoffman, R.M. Widespread skeletal metastatic
potential of human lung cancer revealed by green fluorescent protein
expression. Cancer Res. 58, 4217-4221, 1998.
7. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T.,
Shimada, H., Moossa, A.R., and Hoffman, R.M. A fluorescent orthotopic bone
metastasis model of human prostate cancer. Cancer Res. 59, 781-786, 1999.
8. Hoffman, R.M. Orthotopic transplant mouse models with green fluorescent
protein-expressing cancer cells to visualize metastasis and angiogenesis.
Cancer and Metastasis Reviews 17, 271-277, 1999.
9. Hoffman, R.M. Green fluorescent protein to visualize cancer progression
and metastasis. Methods in Enzymology, Green Fluorescent Protein, Vol. 302.
Ed: P. Michael Conn, Academic Press, San Diego, pp. 20-31, 1999.
10. Naumov, G.N., Wilson, S.M., MacDonald, I.C., Schmidt, E.E., Morris,
V.L., Groom, A.C., Hoffman, R.M., and Chambers, A.F. Cellular expression of
green fluorescent protein, coupled with high-resolution in vivo
videomicroscopy, to monitor steps in tumor metastasis. Journal of Cell
Science 112, 1835-1842, 1999.
11. Yang, M., Chishima, T., Baranov, E., Shimada, H., Moossa, A.R., and
Hoffman, R.M. Green fluorescent protein: A new light to visualize metastasis
and angiogenesis in cancer. Proc. of SPIE Conference on Molecular Imaging:
Reporters, Dyes, Markers and Instrumentation 3500, 117-124, 1999.
12. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S.,
Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M.
Genetically fluorescent melanoma bone and organ metastasis models. Clinical
Cancer Res. 5, 3549-3559, 1999.
13. Yang, M., Chishima, T., Wang, X., Baranov, E., Shimada, H., Moossa,
A.R., and Hoffman, R.M. Multi-organ metastatic capability of Chinese hamster
ovary cells revealed by green fluorescent protein (GFP) expression. Clinical
& Experimental Metastasis, 17, 417-422, 1999.
14. Hoffman, R.M. Visualization of metastasis in orthotopic mouse models
with green fluorescent protein. In: Relevance of Tumor Models in Anticancer
Drugs Development. Eds.: Fiebig, H.H., Burger, A.M., Kluwver Academic
Publishers, Dordrecht, The Netherlands, Vol. 54, 81-87, 1999.
15. Yang, M., Baranov, E., Jiang, P., Sun, F-X., Li, X-M., Li, L., Hasegawa,
S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R.,
Penman, S., Hoffman, R.M. Whole-body optical imaging of green fluorescent
protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97,
1206-1211, 2000.
16. Dove, A. Illuminating cancer models. Nature Biotechnology 18, 261, 2000.
17. Hoffman, R.M. Correspondence re: Initial stages of tumor cell-induced
angiogenesis: Evaluation via skin window chambers in rodent models. Journal
of the National Cancer Institute 92, 1445, 2000.
18. Yang, M., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M.
External optical imaging freely-moving mice with green fluorescent
protein-expressing metastatic tumors. Proc. of SPIE Proceedings 3921,
256-259, 2000.
19. Corrigendum, BioFeedback. Biotechniques 29, 544, September 2000.
20. Yang, M., Baranov, E., Moossa, A.R., Penman, S., Hoffman, R.M.
Visualizing gene expression by whole-body fluorescence imaging. Proc. Natl.
Acad. Sci. USA 97, 12278-12282, 2000.
21. Hasegawa, S., Yang, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman,
R.M. In vivo tumor delivery of the green fluorescent protein gene to report
future occurrence of metastasis. Cancer Gene Therapy 7, 1336-1340, 2000.
22. Rashidi, B., Yang, M., Jiang, P., Baranov, E., An, Z., Wang, X., Moossa,
A.R., and Hoffman, R.M. A highly metastatic Lewis lung carcinoma orthotopic
green fluorescent protein model. Clinical & Experimental Metastasis 18,
57-60, 2000.
23. Bouvet, M., Yang, M., Nardin, S., Wang, X., Jiang, P., Baranov, E.,
Moossa, A.R., Hoffman, R.M. Chronologically-specific metastatic targeting of
human pancreatic tumors in orthotopic models. Clinical & Experimental
Metastasis 18, 213-218, 2000.
24. Yang, M., Baranov, E., Li, X-M., Wang, J-W., Jiang, P., Li, L., Moossa,
A.R., Penman, S., Hoffman, R.M. Whole‑body and intravital optical imaging of
angiogenesis in orthotopically implanted tumors. Proc. Natl. Acad. Sci. USA
98, 2616-2621, 2001.
25. Robinson, K. Imaging system captures whole-body GFP images. Biophotonics
International, April, 54-55, 2001.
26. Pfeifer, A., Kessler, T., Yang, M., Baranov, E., Kootstra, N., Cheresh,
D.A., Hoffman, R.M., and Verma, I.M. Transduction of liver cells by
lentiviral vectors: Analysis in living animals. Molecular Therapy 3,
319-322, 2001.
27. Hoffman, Robert M. Visualization of GFP-expressing tumors and metastasis
in vivo. BioTechniques 30, 1016-1016, 2001.
28. Hutchinson, E. Fluorescence imaging of tumour metastasis and
angiogenesis. The Lancet Oncology, 2, 254, 2001.
29. McCann, J. Jellyfish protein gives new glow to tumor imaging. Journal of
the National Cancer Institute 93, 976-977, 2001.
30. Lee, N.C., Bouvet, M., Nardin, S., Jiang, P., Baranov, E., Rashidi, B.,
Yang, M., Wang, X., Moossa, A.R., and Hoffman, R.M. Antimetastatic efficacy
of adjuvant gemcitabine in a pancreatic cancer orthotopic model. Clinical &
Experimental Metastasis 18, 379-384, 2001.
31. Kamiyama, M., Ichikawa, Y., Ishikawa, T., Chishima, T., Hasegawa, S.,
Hamaguchi, Y., Nagashima, Y., Miyagi, Y., Mitsuhashi, M., Hyndman, D.,
Hoffman, R.M., Ohki, S., and Shimada, H. VEGF-receptor antisense therapy
inhibits angiogenesis and metastasis peritoneal dissemination of human
gastric cancer in nude mice. Cancer Gene Therapy, 9, 197-201, 2002.
32. Li, X-M., Wang, J-W., An, Z., Yang, M. Baranov, E., Jiang, P., Sun, F-X.,
Moossa, A.R., and Hoffman, R.M. Optically-imageable metastatic model of
human breast cancer. Clinical & Experimental Metastasis, 19, 347-350, 2002.
33. Bouvet, M., Wang, J-W., Nardin, S.R., Nassirpour, R., Yang, M., Baranov,
E., Jiang, P., Moossa, A.R., and Hoffman, R.M. Real-time optical imaging of
primary tumor growth and multiple metastatic events in a pancreatic cancer
orthotopic model. Cancer Research 62, 1534-1540, 2002.
34. Yang, M., Baranov, E., Wang, J-W., Jiang, P., Wang, X., Sun, F-X.,
Bouvet, M., Moosa, A.R., Penman, S., and Hoffman, R.M. Direct external
imaging of nascent cancer, tumor progression, angiogenesis, and metastasis
on internal organs in the fluorescent orthotopic model. Proc. Natl. Acad.
Sci. USA 99, 3824-3829, 2002
35. Schmitt, C.A., Fridman, J.S., Yang, M., Baranov, E., Hoffman, R.M., and
Lowe, S.W. Dissecting p53 tumor suppressor functions in vivo. Cancer Cell,
1, 289-298, 2002
36. Hoffman, R.M., Green Fluorescent Protein Imaging of Tumor Cells in Mice.
Lab Animal, 31, 4, 34-41, 2002.
37. Schmitt, C.A., Fridman, J.S., Yang, M., Lee, S., Baranov, E., Hoffman
R.M., and Lowe, S.W. A Senescence Program Contolled by p53 and p16INK4a
Contribures to the Outcome of Cancer Therapy. Cell, 109, 335-346, May 3,
2002.
38. Hoffman, R.M. Watching real-time metastasis in vivo. Trends in Molecular
Medicine, 8(7), 354-355, 2002.
39. Hoffman, R.M. Whole-body fluorescence imaging with Green Fluorescence
Protein. In: Hicks, B., ed. Green Fluorescent Protein Applications and
Protocols: Methods in Molecular Biology. Totowa, NJ: Humana Press, 135-148,
2002.
40. Hoffman, R. M., In vivo imaging of metastatic cancer with fluorescent
proteins. Journal of Cell Death and Differentiation. 9, 786-789, 2002.
41. Zhou, J-H., Rosser, C.J., Tanaka, M., Yang, M., Baranov, E., Hoffman R,m.,
Benedict, W.F. Visualizing sperficial human bladder cancer cell growth in
vivo by green fluorescent protein expression. Cancer Gene Therapy 9,
681-686, 2002.
42. Hoffman, R.M. Green fluorescent protein imaging of tumor growth,
metastasis, and angiogenesis in mouse models. Lancet Oncology 3, 546-556,
2002.
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